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Cell Culture Tubes

In the field of cellular biology and medical research, cell culture tubes play a crucial role. Used for storing and maintaining living adherent or non-adherent cell cultures, these tubes must adhere to strict standards of sterility and non-toxicity. Free from detectable DNase and RNase, human DNA, and pyrogens, cell culture tubes from SECO meet the highest standards of quality.


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Cell Culture Tube Closures

Cell Culture Tube Closures

Glass Cell Culture Tubes

Glass Cell Culture Tubes

Plastic Cell Culture Tubes

Plastic Cell Culture Tubes

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Plastic vs. Resin vs. Glass

As you know, borosilicate glass is the least reactive. It's the most common type of cell culture tubes.


Typically made from plastic resins such as polystyrene, polyethylene, or polypropylene, many of our tubes are surface-treated to promote cell attachment by reducing the inherent hydrophobicity of the plastic.


We also offer specialized cell culture tubes, such as flat-sided ones designed for easy microscopy and optimal attached growth, and tubes suitable for shaking cell suspensions and growing aerobic bacteria. For more advanced applications, we offer tubes designed to create a small bioreactor environment, complete with vented caps for gas exchange.

  • Variety of volume capacities,
  • Variety of materials,
  • Relative Centrifugal Force (RCF) ratings
  • Various closure types.
  • Autoclavable,
  • Designed for either adherent or suspended cell cultures.
How to transfer cells from cell culture plates into cell culture tubes?

Transferring cells, for example HaCaT (a line of human keratinocytes) from cell culture plates into tubes without damaging the cells is a delicate process, which requires careful handling and appropriate technique.


Here are our 6 steps to success.

  1. Cell detachment: Use a cell-specific enzyme like Trypsin-EDTA to detach the cells from the plate. Incubate the culture plate in a controlled environment until the cells detach. Watch for changes under the microscope to prevent over-exposure to trypsin which can harm the cells.
  2. Cell suspension: After the cells are detached, add an appropriate amount of culture medium to neutralize the trypsin. Gently pipette up and down to create a uniform cell suspension.
  3. Cell transfer: Carefully transfer the cell suspension into a centrifuge tube using a sterile pipette. Avoid creating bubbles, as this can damage the cells.
  4. Cell centrifugation: Centrifuge the cell suspension at low speed to pellet the cells.
  5. Supernatant removal and resuspension: Discard the supernatant carefully without disturbing the cell pellet. Resuspend the cells in fresh culture medium.
  6. Final transfer: Transfer the required volume of cell suspension to the cell culture tube using a pipette.
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